2014-2017年河南省副猪嗜血杆菌分离菌株的PCR鉴定及分型

doi:10.11843/j.issn.0366-6964.2019.08.014

Abstract

Abstract: In order to investigate the prevalence of Haemophilus parasuis (HPS) and its serotype in Henan province during 2014-2017, 813 suspected HPS samples were collected from different areas, such as Shangqiu, Luoyang, Zhoukou, etc. Firstly, pathogens were isolated by TSA medium, then, the isolates were identified based on 16S rDNA sequences and were serotyped by type-specific primers. Three different serotype isolates were selected as representative strains, which outer membrane proteins (OMP) were cloned and analyzed. The results showed that the total isolation rate of HPS was 7.75%(63/813), and the isolation rate was from 5.49% (13/237) to 11.31% (19/168) in different years. In these isolates, the serotype 4, 5, 14 and nontypeable strains were 38.10% (24/63), 33.33% (21/63), 7.94% (5/63), 20.63% (13/63), respectively. Serotype 4 and serotype 5 were the most predominant serotypes, followed by serotype 14 and non-typeable strains. Based on OMP sequence analysis, it showed that the homology of nucleotides and amino acids were 98.6%-99.9% and 99.0%-100.0%, respectively. Phylogenetic analysis indicated that all the strains were divided into two groups, Henan-HPS-KF4 and Henan-HPS-LY14 lied in the same branch, Henan-HPS-ZK5 was located in another branch. The comparison of amino acid sequence showed that the 57^th amino acid of Henan-HPS-ZK5 was mutated from T⁵⁷ (Thr) to A⁵⁷ (Ala). The 89^th,115^th,233^th and 239^th amino acid of serotype 4,serotype 5 and serotype 14 HPS were Gln (Q), Glu (E), Arg (R) and Gly (G), respectively, while the other serotypes HPS were Arg (R), Lys (K), Gly (G) and Arg (R), except for the nontypeable strains. These data could respond the epidemiological situation of HPS and its serotypes in Henan province, which will contribute to vaccine research and provides a scientific and objective basis for prevention and control HPS disease.

CLC Number:

S852.612

QIU Jingjing, DING Ke, YU Zuhua, JIA Yanyan, ZHANG Chunjie, WANG Yang, ZHAO Zhanqin. PCR Identification and Serotyping of Haemophilus parasuis Isolated from Henan Province in 2014-2017[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1649-1657.

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PCR Identification and Serotyping of Haemophilus parasuis Isolated from Henan Province in 2014-2017

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